FEBS Lett 1991 Jul 29;286(1-2):159-62

Published erratum appears in FEBS Lett 1991 Nov 18;293(1-2):236

Identification and nucleotide sequence determination of the gene responsible for Ogawa serotype specificity of V. cholerae 01.

Ito T, Ohshita Y, Hiramatsu K, Yokota T

Department of Bacteriology, Faculty of Medicine, Juntendo University, Tokyo, Japan.

The gene encoding a protein of 27 kDa, which is specifically expressed in Vibrio cholerae of serotype Ogawa, was identified and its nucleotide sequence determined. The plasmid carrying this gene was found to convert serotype specificity from Inaba to Ogawa when introduced into the Escherichia coli DH5(pVCI112) cell which harbors a cloned 20-kilobase genomic DNA fragment of V. cholerae NIH35A3 and expresses the 01 antigen of Inaba serotype.